Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. was molecularly diagnosed using Polymerase chain reaction (PCR) technique. Each tested for production catalase enzyme, oxidase analysis starch, analyze gelatin, motility, where Bacillus subtilis positive all tested. Fungal inhibition tests performed by dual culture method agar well diffusion Among them, Azospirillum exhibited potent to growth (72.14% 66.42%) respectively, followed B.pumillus, P.fluorescens, B. Azotobacter ( 58.28%, 43.23%, 39.71% 35.71%) respectively compared with control treatment (0.0%).The dry weight fungus biomass decreased P. fluorescens, sp, subtilis, pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, 0.107 g) that 0. 665 g.